Peptide Bond Formation: Activating Carboxylic Acids to Form Amides (DCC/HATU Logic)
Learn peptide coupling: carboxyl activation, amine attack, and why coupling reagents prevent unfavorable direct condensation. Includes common side reactions and fixes.
Reaction: Amino acid + amino acid → dipeptide
What it does: Forms amide (peptide) bond efficiently
Typical reagents: DCC (often with HOBt) or modern reagents like HATU/EDC